采用聚合酶链式反应(PCR)技术[4]对支原体16s rRNA基因保守区的特异性片段进行扩增检测。
简便、快捷的支原体检测操作:
参考文献:
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[2]. Lincoln, C.K. and M.G. Gabridge, Cell culture contamination: sources, consequences, prevention, and elimination. Methods Cell Biol, 1998. 57: p. 49-65.
[3]. Uphoff, C.C. and H.G. Drexler, Comparative PCR analysis for detection of mycoplasma infections in continuous cell lines. In Vitro Cell Dev Biol Anim, 2002. 38(2): p. 79-85.
[4]. Uphoff, C.C. and H.G. Drexler, Detecting Mycoplasma contamination in cell cultures by polymerase chain reaction. Methods Mol Med, 2004. 88: p. 319-26.